Bivalent DNA vaccine induces significant immune responses against infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus in rainbow trout

DNA immunization and viral load in vaccinated trout following experimental infection with infectious pancreatic necrosis virus (IPNV)

Early Kinetics of Infectious Hematopoietic Necrosis Virus (IHNV) Infection in Rainbow Trout

—A series of experiments was carried out with infectious hematopoietic necrosis virus (IHNV; 193-110 isolate) in rainbow trout Oncorhynchus mykiss (weight, ;1.2 g) to determine the duration of the patent period and the timing of onset of the infectious periods. We first attempted to transmit IHNV to recipient fish from infected rainbow trout 2–3 d after they had been exposed. No infection trans...

Comparative pathogenesis of three strains of infectious hematopoietic necrosis virus in rainbow trout Oncorhynchus mykiss

Three strains of infectious hematopoietic necrosis virus (IHNV) were compared for their pathogenicity to alevin rainbow trout Oncorhynchus n~ykiss by waterborne exposure of the trout to approximately equal concentrations of each virus strain The Idaho strain of IHNV (Type 2) was the most virulent and induced a 62 % mortality over a 10 d period at water temperatures of 10°C. In contrast, strains...

Plaquing procedure for infectious hematopoietic necrosis virus.

A single overlay plaque assay was designed and evaluated for infectious hematopoietic necrosis virus. Epithelioma papillosum carpio cells were grown in normal atmosphere with tris(hydroxymethyl)aminomethane- or HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid)-buffered media. Plaques were larger and formed more quickly on 1- to 3-day-old cell monolayers than on older monolayers. Cell ...

Detection of rainbow trout antibodies to infectious pancreatic necrosis virus by an immunoassay

An enzyme-linked immunosorbent assay (ELISA) for the detection of infectious pancreatic necrosis virus (IPNV) antibodies in rainbow trout Oncorhynchus mykjss was assessed. There was close agreement between the results of the ELISA and a neutralisation test for detecting lPNV antibody when fish sera were tested by both methods. The differences between the ELISA and virus neutralisation are most ...